Life Technologies (Invitrogen)
Rabbit anti-Connexin43
71-0700
Browse Similar Products: Connexin43 Antibodies
In these experiments, I wanted to investigate whether a novel receptor (for olfactory bulb tissue) was co-expressed with known subpopulations - the antibodies used target known histological markers for these subpopulations. Here, I wanted to quantify the protein expression of connexin43 in brain tissue via immunoblot. I used a LI-COR Odyssey to view the blots. This antibody gave good visualization with little antibody used. If the antibody was stored with sodium azide, it could be re-used on a separate blot.
Western Blot
Brain tissue homogenate
1:500 overnight at 4 degrees C
Odyssey Blocking Buffer+Tween-20
1:10,000 for 90 minutes at room temperature
No tertiary incubation
Near-infrared fluorescence
Generated a single band around 43 kDa with low background. Gave quantifiable results.
None
Worked well the first time with minimal fuss.
Can be expensive to buy continually.
Good antibody that does what it is supposed to!