Good ELISA kit for starters

University of California, Davis
Internal Medicine
Graduate Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

LEGEND MAX™ Mouse IL-23 (p19/p40) ELISA Kit with Pre-coated Plates

Catalog Number:

433707

I used this plate for the first time as a graduate student learning various molecular biology and immunology techniques. The plate is simple to use. I use this plate in our lab on in vivo mouse models to check their levels of IL-23.

Experimental Design and Results Summary

Application

ELISA to determine serum/urine levels in in vivo mouse models.

Starting Material

Serum/urine

Protocol Overview

As per the kit

Tips

Nothing really. It's very simple and easy to use. I would say user friendly.

Results Summary

Could determine very high levels in the mice. I am not sure about the lowest detection limit though as my experiment generally should result in high signal. Overall, there was very low background and wells were specific for the results.

Additional Notes

None

Image Gallery

Summary

The Good

User friendly. Follow instructions and you are done.

The Bad

Can't say yet.

The Bottom Line

Good kit for new learners in the field. Zero background at times is one extremely good thing I liked about this kit.

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