Biorbyt
CX3CR1 antibody from Biorbyt
orb67205
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The long-term goal of our lab is to understand the molecular and genetic mechanisms underlying brain development and disases. My work is to study the role of microglia in axon regenreation upon injury. CX3CR1 is a cell surface receptor highly expressed in microglia, while CCR2 is a cell surface receptor highly expressed in its precursor monocyte. Therefore, the CX3CR1/CCR2 ratio represent the differentiation of monocyte to functional microglia. To established an in vitro model of microglia, this product was used to trace the differentiation of monocytes to microglia, cells were stained for CX3CR1 and CCR2 together with DNA marker DAPI. The high CX3CR1/CCR2 ratio suggests microglia identify.
Immunofluorescence
Microglial cells
1:1000 at 4°C for 12 hours
3% BSA and 0.1% Triton 100 in PBS buffer
1:2000 for 1 hours at 23°C
NA
ECL method
The high CX3CR1/CCR2 expression ratio suggests differentiated microglia identify.
None
strong signal and specific
This antibody is suitable for immunocytochemistry.