PE/Cy7 anti-mouse CD8a Antibody for CD8 T cell staining

Cancer Biology
Scripps research center in FL
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse CD8a Antibody

Catalog Number:

100722

Naive spleen cells were collected from a mouse and stained with this antibody.  During our experiments (including those run using this antibody), we used a number of different antibodies to look at the phenotype change of CD4 T cells. For example, if we want to look at CD62L subpopulation in CD4 Tcells, if CD62L is PE-conjugated, then we have to use CD4 FITC-conjugated, or PE-Cy7-conjugated (at least not PE channel). These are always dependent on the color set.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:200 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

Around 20% of the cells were CD8 positive.

Additional Notes

None

Related Categories

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Summary

The Good

Easy application

The Bad

None

The Bottom Line

Can be used to gate CD8+ T cell population.

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