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PE anti-mouse CD4 Antibody
100408
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Mouse spleen was collected and stained with this antibody. During our experiments (including those run using this antibody), we used a number of different antibodies to look at the phenotype change of CD4 T cells. For example, if we want to look at CD62L subpopulation in CD4 Tcells, if CD62L is PE-conjugated, then we have to use CD4 FITC-conjugated, or PE-Cy7-conjugated (at least not PE channel). These are always dependent on the color set.
Flow Cytometry
Mouse spleen
1:300 dilution
PBS
N/A
FACS LSRII
Around 30% of the cells were determined to be CD4 +.
None
Easy application
Can be used to gate CD4 + T cell population.