APC anti-mouse CD80 Antibody for CD80 staining on DCs

May 04, 2015

Cancer Biology
Scripps research center in FL
Research Associate

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Company:

BioLegend

Product Name:

APC anti-mouse CD80 Antibody

Catalog Number:

104714

Info: View Product View Product Specs

Browse Similar Products: CD80 Antibodies

Mouse bone marrow was isolated and cultured in vitro with GM-CSF for 5 days, and stained with anti-mouse CD11c Antibody and this Ab.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Bone Marrow Derived DC

Primary Incubation

1:200 dilution

Blocking Agent

PBS

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS LSRII

Results Summary

After 5 days culture, differentiated DC cells had expression of CD80 (Green line, red line is isotype control)

Additional Notes

None

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Summary

The Good

Easy application

The Bad

NA

The Bottom Line

Can be used to detect CD80 expression on DCs

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