Brilliant Violet 605™ anti-mouse CD25 Antibody for Treg staining

January 28, 2015

Cancer Biology
Scripps research center in FL
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-mouse CD25 Antibody

Catalog Number:

102036

Naive spleen cells were collected from a mouse and stained with CD3CD4 and this antibody

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:200 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

Only a small population of CD25+ T cells are in CD3CD4 T cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy application

The Bad

None

The Bottom Line

Can be used to detect Treg cell population.

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