Integrin a4ß7 expression on naive CD4 T cells

January 29, 2015

Cancer Biology
Scripps research center in FL
Research Associate

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Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse LPAM-1 (Integrin α4β7) Antibody

Catalog Number:

120606

Info: View Product View Product Specs

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Naive spleen cells were collected from a mouse and stained with CD3CD4 and this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:200 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

Only tiny expression of Integrin α4β7 can be detected on naive CD3CD4 T cells from the mouse spleen

Additional Notes

None

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Summary

The Good

Easy application

The Bad

None

The Bottom Line

Can be used to evaluate the expression of Integrin α4β7 on T cells

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