Western blot review for anti-histone H3 (mono methyl K4) antibody

January 15, 2015

Cardiology
Mount Sinai
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Histone H3 (mono methyl K4) antibody

Catalog Number:

ab8895

This product was used to determine if changes in H3H4me1 are associated with endothelial-mesenchymal-transition. This antibody was recommended to me by a co-worker.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Isolated chromatin from untreated HUVECs and treated with TGFb2.

Primary Incubation

1:1000 dilution in 5% skim milk in TBS-t overnight at 4 degrees

Blocking Agent

5% skim milk in TBS-t for 1 hour at room temperature

Secondary Incubation

1:10,000 in 5% skim milk in TBS-t for 2 hours at room temperature (anti-rabbit Thermo Scientific Cat#31460)

Tertiary Incubation

NA

Detection

Enhanced chemiluminescence

Results Summary

Band detected at 15kDa which showed decreased expression in HUVECs after treatment with TGFb2. Exposure time of 10 seconds with film.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

This antibody detected a single band, very clean Western

The Bad

None

The Bottom Line

Would definitely recommend this antibody

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