PE-CF594 anti mouse RORgamma-t for Th17 cells

Cancer Biology
Scripps research center in FL
Research Associate

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Company:

BDBiosciences

Product Name:

PE-CF594 anti mouse RORγt

Catalog Number:

562684

Browse Similar Products: PE-CF594 Flow Cytometry Antibodies

We transfer wild type CD4 T cells to Rag-/- mice to establish a Colitis model and then look at the phenotype of these transferred CD4 T cells. RORγt is required for the differentiation of Th17 cells and expression of IL-17. This Ab can be used to evaluate the IL-17 production in RORγt+ and RORγt- CD4 T cell population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:300 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

RORγt is required for the differentiation of Th17 cells and expression of IL-17. This Ab can be used to evaluate the IL-17 production in RORγt+ and RORγt- CD4 T cell population.

Additional Notes

None

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Summary

The Good

Easy application

The Bad

None

The Bottom Line

Easy handling and can be used at low concentration

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