APC Annexin V in combination with Bv506 viability dye

December 30, 2014

Cancer Biology
Scripps research center in FL
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC Annexin V

Catalog Number:

640920

Annexin V was used for its function as an apoptosis marker.  APC Annexin V doesn't have to be used with PI viability dye  and it can be used in combination with Bv506 viability dye.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:20 dilution

Blocking Agent

APC Annexin V binding buffer

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS LSRII

Results Summary

Surface staining including viability dye must be performed first in PBS, and then washed with APC Annexin V binding buffer. Cells can be stained with APC Annexin V (1:20) in APC Annexin V binding buffer.

Additional Notes

None

Related Categories

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Summary

The Good

Easy application

The Bad

Needs high concentration. for each sample (1million to 10million), it needs 100ul staining buffer with 5ul of APC Annexin V.

The Bottom Line

Easy application and can be used together with other viability dyes besides PI.

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