Novus Biologicals
TRAF7 Antibody
NB600-1399
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This Western blot was performed to examine if Traf7 is expressed in HUVEC (Human Umbilical Vein Endothelial Cells). Molecular weight of TRAF7 is about 75KDa. It is not detected.
Western Blot
HUVEC (Human Umbilical Vein Endothelial Cells)
0.5-2ug/ml in TBST with 5% milk, overnight in cold room
TBST with 5% milk
Donkey anti-rabbit IgG-HRP 1:5000 in TBST with 5% milk, 1hr at room temp
No tertiary incubation was performed
ECL
Molecular weight of TRAF7 is about 75KDa. It is not detected.
None
None.
Not working.
This is not a good antibody for WB.