Sigma-Aldrich
Mouse anti-Lis1
L7391
Browse Similar Products: anti-Lis1 Antibodies
This antibody has been extensively used to detect endogenous and overexpressed Lis1 in wild type, Lis1 knock-out and TgLis1 mouse tissues to confirm identity of transgenic samples. It has also been used in protein-protein interaction studies to detect presence of Lis1 in protein complexes following immunoprecipitaion. While the antibody is very expensive, of all anti-Lis1 antibodies tested this one provides the cleanest signal, can be used at lowest concentration and detects lowest amount of Lis1 protein. So ultimately, it is in fact the cheapest on a per experiment basis.
Western Blot
Whole brain lysate, NIH/3T3 and HEK293 cell lysates. Total protein can be as low as 0.25 mg/ml.
Overnight at +4C
1% non-fat dry milk in 1x TBS + 0.05% Tween20
One hour at room temperature
NA
SuperSignal Pico (Pierce/Thermo) or secondary antibody conjugated to IRDye800
The antibody detects a single band of the expected size around 45 kDa
None
Specificity, high affinity
Price, does not work in immunofluorescence
The best anti-Lis1 antibody for Western Blot