Cell Signaling
Rabbit anti Phospho Pin1 (ser16)
#3721
Browse Similar Products: Phospho Pin1 (ser16) Antibodies
This product is one of few phospho antibodies on the market for Pin1. It comes as 50ul but about a 1:100 to 1:500 dilution is necessary to detect phosphorylation reliably. The blot is often dirty, likely due to non specificity, but no bands other than Pin1 appear phosphorylated. Recognizes all listed host species as well as opossum Pin1.
Western blot
Stimulated Human Neutrophil Lysate
1:500 overnight at 4 degrees in Licor Blocking Buffer
5% Milk in TTBS
Gt anti Rabbit IgG Alexa Fluor 700, 1:5000, 1.5 hours at room temperature
3 washes with TTBS 5' each
Fluorescent Western Imager (Li-Cor Oddysey)
The blot was blurry, but bands were easily detectable and densitometry was performed with ease. Obtaining publication quality images may be an issue.
None
It works and it's available.
It is a weak signal that is often dirty.
With not many options available this product is necessary for measuring Pin1 Phosphorylation by Western blot.