Alpha-actinin antibody for cardiomyocyte staining

December 10, 2014

Cardiology
BIDMC
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Sigma

Product Name:

Alpha-actinin antibody

Catalog Number:

A5044

Browse Similar Products: Alpha-actinin Antibodies

Excellent Antibody to characterize human iPSC-derived cardiomyocytes in cell culture.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human iPSC-derived cardiomyocytes

Primary Incubation

Dilution 1/500 in 5% milk with 0.1% Triton

Blocking Agent

5% milk with 0.1% Triton

Secondary Incubation

Anti-mouse alexa 594 antibody

Tertiary Incubation

NA

Detection

Immunofluorescence

Results Summary

Very specific staining of cardiomyocytes with a one hour incubation at room temperature.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specificity, short time, inucubation

The Bad

None

The Bottom Line

Excellent antibody for the staining of human cardiomyocytes in culture. Also works perfectly for neonatal rat cardiomyocytes.

Share your experience with other scientists. Write a Review! »

Join the discussion