BUV395 amazing addition in the multicolor panel

CVD
Mayo Clinic
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BDBiosciences

Product Name:

BUV395 CD19 and BUV395 B220

Catalog Number:

563557

Browse Similar Products: CD19 Flow Cytometry Antibodies

563793 and 563557 BUV provides a new addition to the multi-color flow cytometry panel. I have tried both Anti-mouse CD19 and CD45R (B220) with this channel and both work extremely well . As recommended by BD, this dye works with the BV dyes in the BV buffer. The BD recommended filter is a MUST (Usually BD is kind enough to send this filter as a gift sample) if you are using it with BV421.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse liver and kidney

Primary Incubation

45 minutes

Blocking Agent

FCR block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSRII

Results Summary

Very dependable in a multi-color panel. With right filters easily compensates with colors exited by violet, blue and red lasers. Works well with all the BV dyes.

Additional Notes

None

Image Gallery

Summary

The Good

Ease of compensation

The Bad

Limited antibody options available. In my hands, anti-mouse CD11b did not work well.

The Bottom Line

Dim dye but dependable as a dump channel. Ease of compensation.

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