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December 12, 2014

mol and Human Bio
BCM
lab manager

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Company:

Santa Cruz Biotechnology

Product Name:

GAPDH Antibody (6C5)

Catalog Number:

sc-32233

Browse Similar Products: GAPDH Antibodies

Most of the antibodies are normalized with respect to GAPDH. This antibody is very specific and particularly inexpensive. It works both with human and mouse protein extracts in Immunofluroscence and Western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue protein extracts

Primary Incubation

1:5000

Blocking Agent

5% milk in TBST

Secondary Incubation

Anti- mouse in blocking solution

Tertiary Incubation

NA

Detection

Chemiluminiscent substrate

Results Summary

GAPDH works very specific at 44Kda and its used to normalize the expression data. One hour incubation with this primary antibody is enough to produce sufficient signal. It can even be co-incubated with other antibodies with different sizes of expression.

Additional Notes

None

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Summary

The Good

Very inexpensive and lasts a long time

The Bad

Has a discrepancy with nuclear and cytoplasmic fraction of protein extracts.

The Bottom Line

This antibody can make reproducible data and can be used more often than tubulin and actin due to its specificity. Overall a good normalizing antibody and highly reliable one.

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