Bio-Rad
InstaGene
732-6030
Info: Supplier Page
When screening samples of varying composition, such as complex foods, obtaining a purified DNA template with fewer active inhibitors is advantageous over traditional template preparations such as boiling. This is why our lab often uses InstaGene to prepare DNA templates for amplification reactions such as PCR. The quick protocol and low cost of the reagent are also key factors making this a popular product.
DNA template preparation
Pure bacterial cultures or inoculated food samples
Isolate bacteria in a sample by centrifugation, add 200 ul of InstaGene matrix to the sample, incubate at 55 degrees C for 20 minutes, boil for 8 minutes, centrifuge and keep supernatant.
100 ul of matrix is often enough, and 2 ul of template is often sufficient for amplification.
Amplification is always observed if target bacteria is present in the sample, no contamination has been seen in InstaGene negative controls.
Includes a mini-stir-bar in the bottle.
Quick and easy protocol, also a cheaper reagent.
None
Well worth the purchase.