Excellent Ab for IL-4 intracellular staining

Cancer Biology
Scripps research center in FL
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse IL-4 Antibody

Catalog Number:

504104

This Ab was used to detect intracellular staining of IL4 from mouse CD4 T cell differentiated Th2 cells in vitro.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse cultured CD4 T cells

Primary Incubation

1:200 diluation, 15 minutes in ice after fixation and permerblization (PFA).

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

Can be easily used to stain intracellular IL4 production in differentiated Th2 cells.

Additional Notes

None

Related Categories

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Summary

The Good

Easy usage even at low concentration 1:300, super bright

The Bad

None

The Bottom Line

Very good Ab for intracelluar staining of IL-4 in vitro if the differentiation is good.

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