Excellent Ab for IL-17 intracellular staining

Cancer Biology
Scripps research center in FL
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse IL-17A Antibody

Catalog Number:

506908

Info: View Product View Product Specs

Browse Similar Products: IL-17 Antibodies

This Ab was used to detect intracellular staining of IL17 from mouse CD4 T-cell differentiated pathogenic Th17 cells in vitro.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse cultured CD4 T cells

Primary Incubation

1:200 diluation 15 minutes in ice after fixation and permerblization (PFA)

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

Can be used to stain intracellular production of IL-17 after fixation and permerblization with PFA related buffer.

Additional Notes

None

Related Categories

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Summary

The Good

Easy usage even at low concentrations 1:200

The Bad

Must use PFA related buffer, commerical available buffer for foxp3 is not working

The Bottom Line

Can easily stain Th17 cells that are secreting IL-17.

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