Excellent Ab for intracellular staining of mouse IFN-g

November 06, 2014

Cancer Biology
Scripps research center in FL
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse IFN-γ Antibody

Catalog Number:

505809

We are looking at the intracellular staining of mouse IFN-gamma in CD4 memory T cells when stimulated by PMA, Ionomycin and BFA.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse CD4 T cells

Primary Incubation

1:200 for 15 minutes on ice

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

It can easily stain the population secreting IFN-gamma.

Additional Notes

N/A

Related Categories

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Summary

The Good

Excellent Ab for ICS of mouse IFN-gamma.

The Bad

None

The Bottom Line

Can be used together with other Abs including IL-10, IL-17A.

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