CD3 Antibody works at high concentration

November 06, 2014

Cancer Biology
Scripps research center in FL
Research Associate

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Company:

BioLegend

Product Name:

Brilliant Violet 650™ anti-mouse CD3 Antibody

Catalog Number:

100229

We are trying to sort CD3 population in mouse spleen cells by Brilliant Violet 650™ anti-mouse CD3 Antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:20 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

The binding between antibody and CD3 is weak, only higher concentration 1:20 can get good staining.

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Can stain a CD3 population

The Bad

Needs high concentration

The Bottom Line

It can be used to stain mouse spleen cells together with other antibodies including CD4, CD8, CD25, CD44 and CD62L.

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