SA Scientific
Fluorescent Detection Reagent
E-2210
Our lab was conducting Loop-mediated isothermal amplification (LAMP) experiments using both SA scientific kits and bst 2.0 homemade reactions. We wanted to increase the detection ability of the reactions beyond qualitative observations of turbidity. The reagent allows easy qualitative observation under both white light and UV light. The reagent is calcein based.
LAMP detection
LAMP reactions
Add 1 µl of Fluorescent Detection Reagent to each tube or in a master mix prior to the reaction. Visualize tubes under white light or UV after reaction.
NA
Worked well, as expected. The tubes are a clear green shade if the reaction has worked, but remain a pink shade if not. Under UV the tubes are clearly fluorescent if positive or not if negative.
Comes shipped in a microtube with a product insert.
Works as advertised
None
Great reagent to speed up and ease detection of LAMP reactions.