Life Technologies
BACH2 copy number assay taqman probe
Hs00222364_m1
I have used the TaqMan Copy Number probe to assess BACH2 copy number in lymphoma biopsies by RT-PCR
Copy number assessment by qRT-PCR
DNA extracted from lymphoma biopsies
The Life Technologies test assay is labeled with FAM dye, while the reference assay, which must be purchased separately, is VIC labelled. The assays are run in a duplex manner and the reference detects a target sequence known to be present in two copies in the diploid genome. Relative quantification is then calculated using the Life technologies CopyCaller software, which is freely available. Practically, the test and reference assay are combined with 5ng of DNA and genotyping master mix, then run as per a standard qRT-PCR assay. The protocol is very quick to perform and complete within 2-4 hours.
The reference assays must be validated
The results are adequate for validation of CNV analysis by other methods, as well as for quick visualization of CNV estimation.
NA
Only uses a very small amount of DNA
Reference assay requires optimisation
Rapid method to validate copy number changes by RT-PCR.