Santa Cruz Biotechnology, Inc.
MCP-1 (5) Antibody
sc-130328
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MCP-1 protein expression was characterized via Western blot by using purified MCP-1 (C-17) Antibody
Western blot
Cell lysate of human A549 cells
1:500; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3
5% low-fat milk solution
1:5000, 1 h, room temperature, rabbit-anti-mouse IgG – HRP conjugated
ECL Plus Detection Reagents from Amersham
Using the above mentioned antibody (1:500) one band was detected for MCP-1 at approximately 12 kDa.
NA
There was a distinct band at approximately 12 kDa.
Two bands in higher ranges appeared too.
Using the above mentioned antibody (1:500), one band was detected for MCP-1 at approximately 12 kDa via Western blot.