Western blot via p44/42 MAPK (Erk1/2) Mouse mAb from Cell Signaling Technology

November 07, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

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ERK 1/2 protein identified using p44/42 MAPK (Erk1/2) mouse mAB.

Company:

Cell Signaling Technology

Product Name:

p44/42 MAPK (Erk1/2) Mouse mAb

Catalog Number:

4696S

Erk1/2 protein expression was characterized via western blot in HL-1 cells

Experimental Design and Results Summary

Applications

Western blot

Sample

Cell lysate of murine HL-1 cells

Primary Incubation

1:500; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3

Blocking Agent

5% low-fat milk solution

Secondary Incubation

1:5000, 1 h, room temperature, rabbit-anti-mouse IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL Plus Detection Reagents from Amersham

Results Summary

Using the above mentioned mouse antibody (1:500), 3 bands were detected including one for p44/42 MAPK (Erk1/2) at 44 kDa.

Additional Notes

NA

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ERK 1/2 protein identified using p44/42 MAPK (Erk1/2) mouse mAB.

Summary

The Good

Three bands were detected including one for p44/42 MAPK (Erk1/2) at 44 kDa

The Bad

None

The Bottom Line

The antibody worked well. 3 bands were detected including one for p44/42 MAPK (Erk1/2) at 44 kDa.

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