R&D Systems
Human CXCR4 (Fusin) MAb (Clone 12G5)
MAB170
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Western blotting was performed using anti-human CXCR4 (Fusin) antibody in human endothelial cells.
Western Blot
Lysate of human endothelial cells.
1:1000; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3
5% low-fat milk solution
1:5000, 1 h, room temperature, rabbit-anti-mouse IgG – HRP conjugated
ECL Plus Detection Reagents
Using the above mentioned antibody (1:1000), one prominent band was detected for CXCR4 (43 kDa)
NA
Using the above mentioned antibody (1:1000), one prominent band was detected for CXCR4 (43 kDa).
Some other bands were detected too.
This antibody works well for Western blotting.