Life Technologies
SF2/ASF Monoclonal Antibody, Mouse (96)
324500
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SF2/ASF protein expression was characterized via western blot using the SF2/ASF Antibody (dilution 1:1000).
Western Blot
Cell lysate of human endothelial cells
1:1000; over night , 4°C , in TBS-T; 1%BSA; 0.02 Na3
3% BSA solution
1:5000, 1 h, bei room temperature, goat-anti-rabbit IgG, HRP-conjugated
5% low-fat milk solution.
ECL Plus Detection Reagents (Amersham)
Using the above mentioned antibody (1:1000) one band was detected for SF2/ASF at approximately 30 kDa.
NA
SF2/ASF was detected by this antibody in a clear band
This antibody works well for Western blotting.