New England Biolabs
Bst 2.0 DNA polymerase
M0537S
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A loop-mediated isothermal amplification (LAMP) study was conducted to detect the presence of pathogen DNA in food samples. Bst 2.0 from NEB was selected because it is advertised as having improvements from bst, is relatively inexpensive, and is from a reputable enzyme supplier. It was used in conjunction with nucleotides from NEB and a number of additional additives. The enzyme works well, although reactions may require some optimization. Additional magnesium was supplemented into the reaction ( between 2 - 8 mM) by using PCR grade MgCl2 from Ambion.
Loop-mediated isothermal amplification (LAMP) for pathogen DNA detection.
DNA template extracted from samples using Instagene reagent from BioRad, or boiled samples.
Set up a master mix containing: bst 2.0, isothermal amplification buffer (supplied), nucleotides, MgCl2, primers, DNase free water, and betaine.
Start optimization with varying concentrations of MgCl2.
Once optimization was complete, reactions worked well.
Supplied with isothermal amplifications buffer.
Works well after optimization
Requires additional PCR grade Magnesium source
Good enzyme worth the price.
Product Name: Bst 2.0 DNA PolymeraseSupplier Page