Solid workhorse machine

October 07, 2014

Florida State University
Biological Science
Graduate Student

Overall

Performance

Ease-of-Optimization

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Amplification plot from 7500 Fast Real-Time PCR System

Company:

LifeTechnologies

Product Name:

7500 Fast Real-Time PCR System

Catalog Number:

4351107

We wanted to perform qPCR testing to determine if treatment of a G-protein-coupled receptor with an agonist would affect the transcription of different genes. This machine worked well for us over multiple projects and generated a lot of data.

Experimental Design and Results Summary

Application

Quantitative PCR

Starting Material

cDNA from tissue extracts and cell culture extracts

Protocol Overview

Tissue or cells were collected, treated with RNAlater(TM) and stored at -20 degrees C until extraction. RNA was extracted via TRIzol and the RNA was reverse-transcribed using a Superscript 3 kit.

Tips

Does not have a gradient function; must test annealing temperatures in separate experiments.

Results Summary

The machine worked well and consistently generated quality data, even when on for days straight. Plates were run every 2.5 hours for 24 hours, and none of the data from the plates showed any drift. The current software does a good job of identifying the right Ct, though you should still check if it's correct.

Features Summary

Mostly basic features. Competitors have gradient features that this lacks.

Additional Notes

Related Categories

Image Gallery

Amplification plot from 7500 Fast Real-Time PCR System

Summary

The Good

Great machine, consistent.

The Bad

Lacks some features that are common to other machines.

The Bottom Line

If you want a qPCR machine that will keep running at all hours and not fail you, this is a great machine. They have been working on the software, which helps make this a better investment than before.

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