Excellent antibody for immunofluoresence detection of YAP/TAZ in mouse cells

October 02, 2014

Cardiology
BIDMC
Postdoc

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Immunofluorescence of mouse embryonic fibrobalsts (MEFs) stained with anti-YAP/TAZ antibody and Alexa 594 conjugated secondary antibody.

Company:

Cell Signaling

Product Name:

YAP/TAZ (D24E4) Rabbit mAb

Catalog Number:

8418

Info: View Product View Product Specs

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The goal of the study was to detect YAP/TAZ localization in mouse embryonic fibroblasts by immunofluorescence

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse embryonic fibroblasts

Primary Incubation

1/100 dilution at room temperature for 1 hour

Blocking Agent

5% Normal goat serum for 1 hour at room temperature

Secondary Incubation

anti-rabbit alexa 594 at 1/400 dilution for 30 min at room temperature

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

This antibody gives a good signal with a low background. It detects YAP/TAZ mostly in the nucleus as expected when cells are at low density and in proliferation stage.

Additional Notes

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Immunofluorescence of mouse embryonic fibrobalsts (MEFs) stained with anti-YAP/TAZ antibody and Alexa 594 conjugated secondary antibody.

Summary

The Good

Specificity

The Bad

None

The Bottom Line

I highly recommend this antibody to determine localization of YAP/TAZ in culture mouse cells using immunofluorescence.

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