Detecting TGFß1 in Human Sarcoma

Overall

Quality of Results

Ease-of-Optimization

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Cancer Research Program
Fundación IMIM
Lab manager

Company:

Santa Cruz Biotechnology

Product Name:

TGFβ1 (V) Antibody

Catalog Number:

sc-146

The TGF family of proteins are implicated in many tumor processes, such as, development, growth, migration, and metastasis. A good antibody for TGF detection is essential in tumor analysis. Here we show an anti-TGFβ1 antibody that works perfectly in human samples.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human sarcomas

Primary Incubation

1/100

Blocking Agent

H2O2

Secondary Incubation

Anti Rb HRP

Tertiary Incubation

Not needed

Detection

DAB

Results Summary

Nice extracellular detection

Additional Notes

Detection was performed using the CSA II Amplification System (Dako) in a Dako Link platform.

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Summary

The Good

Specificity.

The Bad

Not very intense signal

The Bottom Line

Despite low signal in some samples, we consider it a good option to detect TGF-Beta1 protein.

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