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PE anti-human CD69 Antibody
310905
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The stimulation of T cells is carried out by different methods. In order to check if your stimulation worked or not, there are many factors you can look at. CD69 is one of these markers. Lindsey WB et al (Cytotherapy. 2007;9(2):123-32) show that it's a very good marker.I use this antibody because it's a good antibody, easy to use and relatively cheap.
Flow Cytometry
T cells
Mix with 5uL antibody 30 min on ice
Human IgG
Not required
Flow cytometry
In the example flow plot shown, T cells were either incubated with media only (red histogram) or stimulated with PMA/ionomycin using a standard protocol. CD69 can be seen strongly positive on the PMA-stimulated cells (blue histogram).
Easy to use, relatively cheap, can be used for other cells
None
To check that your stimulation assay worked, staining with this antibody will help you feel more confident about the results.