Cell Signaling
Cleaved PARP (Asp214) Antibody (Mouse Specific)
9544
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Programmed cell death (PCD) is a critical process that underlies normal tissues development and serves as a homeostatic mechanism to maintain cell populations. Importantly, dysregulation of PCD has been demonstrated to promote various disease pathologies and is a contributing factor in the development of most cancers. The main form of PCD is known as apoptosis, which results in the activation of numerous proteolytic enzymes termed caspases. Substrates of caspases include a wide range of host proteins that induce cell death upon proteolytic cleavage. This review focuses on the cleavage of PARP-1 that was utilized as a read-out for apoptotic cell death.
Western blot
Mouse embryonic fibroblasts (MEFs)
5% skim milk in TBST with anti-cleaved PARP 1/1000- 1 hour at room temperature
5% skim milk in TBST- 10 minutes at room temperature
1/10000, room temperature for 1 hour in TBST 5% skim milk
NA
HRP- chemiluminescence
This antibody only recognizes the cleaved form of PARP (~89kDa). Apoptosis was achieved only when MEFS were stimulated with apoptotic stimuli (CHX, cycloheximide and TNF) as cleaved PARP was detected.
Highly specific antibody with no background detection on Western blot.
Mouse specific....but works great!
Another great antibody from Cell Signaling!!