CD41: Very Useful Reagent

Overall

Quality of Results

Ease-of-Optimization

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CSB
MGH
postdoc

Company:

Biolegend

Product Name:

PE/Cy7 anti-mouse CD41 Antibody

Catalog Number:

133916

The aim of the research is to study bone marrow progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected CD41 Antibody from Biolegend specifically because CD41 is expressed in some progenitor cells and can be found in bone marrow. Using this product I was able to differentiate progenitor subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow

Primary Incubation

1:300 30' in ice

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

No need to define the best working concentration. Bright and easy to compensate with PE, 7AAD and APC-Cy7.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Useful for multicolor panels on limited sample amount.

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