BioLegend
Purified anti-TICAM-1 (TRIF) Antibody [Clone: 1H4B01]
657102
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Browse Similar Products: TICAM1 Western Blot Antibodies
We research complex lung disorders.
High levels of inhaled oxygen, or hyperoxia, is commonly used as a life-sustaining measure in critically ill patients, especially neonates. However, prolonged exposures can exacerbate respiratory failure. Several studies showed that TLRs confers protection against hyperoxia-induced lung injury and mortality. TICAM-1 negatively regulates TLR signalling, so we wanted to check whether hyperoxia somehow affects TICAM-1 expression and disrupts TLR signaling. To our knowledge, our studies are the first to define role of PICAM-1-TLR cytoprotective axis in the lung and epithelial cells. This pathway is a potential therapeutic target against a range of oxidant-induced lung injuries.
Western Blot
MLE12 cell line
1:1000, 2 hrs. at Room Temperature
5% Milk in TBST buffer
1:5000 goat anti mouse in 5% Milk for 1 hr. at Room Temperature.
Wash X5 with TBST buffer
GE ECL reagent
I found increased expression of TICAM-1 with hyperoxia up to 24 hrs. Lane-1 Room Air control. Lane-2 Hyperoxia (16 hr) Lane-2 Hyperoxia (24 hr) Lane-2 Hyperoxia (48 hr)
None
Great specificity and cheap price
It is a great antibody for TICAM-1 western blotting.