This kit works very well and yields good quality plasmids suitable for transfection and other downstream applications

Overall

Quality of Results

Ease-of-Optimization

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Virginia Tech, ILSB, Blacksburg, VA
Biomedical science and Pathobiology
Research Scientist

Company:

QIAGEN

Product Name:

QIAGEN Plasmid Midi Kit (25)

Catalog Number:

12143

I was looking for high quality plasmid DNA for transfection studies and used this Qiagen Midikit that worked really well. The quality and yield of plasmid DNA obtained were both good. I recommend this product strongly to others.

Experimental Design and Results Summary

Application

Plasmid DNA isolation

Starting Material

Bacterial culture

Protocol Overview

I was looking for an efficient kit to get good concentration for low copy number plasmids with large size (20kb). I tried several kits in the market, but they were not up to the mark. Qiagen's kit resulted in good quantity and quality of plasmid i was looking for.

Tips

Highly recommended for a large size plasmid

Results Summary

Got a good yield upto 10-12ug/100ml culture.

Additional Notes

None

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Summary

The Good

Gives good yield without impurities for transfection

The Bad

Long protocol

The Bottom Line

It's worth going for this kit when large sized plasmids are being prepared from bacterial cultures that maintain low copies.

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