BioLegend
FITC anti-human CD97 Antibody
336306
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Browse Similar Products: CD97 Flow Cytometry Antibodies
FITC anti-human CD97 Antibody (Biolegend, cat#336306) is a good one. It stains quite well on the cell surface CD97 in living human cancer cells.
Flow Cytometry
Cultured human cancer cell lines
One million cells were incubated with antibody for 30 minutes at 4 degrees C in the dark.
2% FBS in PBS
Secondary antibody was NOT needed since this antibody is FITC conjugated.
NA
FITC signal in flow cytometry.
Several human cancer cell lines were tested, the CD97 expression is relevant to the RNA level of CD97.
Cells were detached from dishes with EDTA without trypsin.
Clean antibody
None
Cells need to be detached by EDTA, not Trypsin, to protect surface protein.