I love BioLegend Brilliant Violet 421, perfect replacement for PB

Overall

Quality of Results

Ease-of-Optimization

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Medicine
University of Florida
Research Assistant

Company:

BioLegend

Product Name:

Brilliant Violet 421 anti mouse/human CD11b

Catalog Number:

101236

Pacific Blue is good because it doesn't have compensation with most used colors like PE, APC, APC-CY7, PE-CY7, and FITC. However, PB is not bright and it was not stable for intracellular staining or fixation or permeabilization. Biolegend BV421 is a perfect replacement for PB. It is stronger and more stable for intracellular staining. 

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Stained myloid cells

Primary Incubation

See data sheet for recommended concentration

Blocking Agent

2% FBS in PBS

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

Works in PB channel.

Additional Notes

None

Related Categories

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Summary

The Good

Stronger, brighter, stable, reasonable price

The Bad

None

The Bottom Line

A must have for flow cytometry

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