Active Motif
KAT6A antibody
61327, 61328
Browse Similar Products: KAT6A Antibodies
In my experiments, we study protein-protein interaction between KAT6A and a KAT6A interacting protein. The antibody gave very nice pull-down, but in Western blot besides the main band it showed several minor bands contributing to 30-40% of the signal. ChIP experiment from odontoblast cells gave only 5-6% binding site pull-down for a specific promoter. I know from my transcriptional data that this promoter is very active at that point. I got specific but lower pull down efficiency.
ChIP
Soluble chromatins from odontoblast cells.
Overnight at 4 with Active Motif ChIP buffer.
NA
Protein beads purification, DNA isolation and QPCR analysis
Only 6-8% pulldown over input DNA.
Specific because no binding in 3'UTR region.
Efficiency was low.
It may be that the affinity of the antibody is lower or you need more than the recommended amount.