ChemidocMP and Software are Very Easy to Use

Overall

Performance

Ease-of-Optimization

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Saint Louis University
Pharm & Phys Science
Post Doc

Company:

Bio-Rad

Product Name:

Chemidoc MP

Catalog Number:

170-8280

We use this product for a variety of imaging purposes but mainly for Western Blot imaging and analysis. It is very easy to use once optimized. Since Bio-Rad has stain free technology, the western blotting procedure needs to be optimized for use with their stain free gels, however it cuts down on a majority of the time and effort downstream. There is no need to strip and reprobe for housekeeping proteins since the imager is capable of normalizing to total lane protein and perform a variety of analyses. The software (image lab) is great, user friendly, license free, and works on Macs and PCs! Overall this is a great system once you optimize it for your needs. The sales reps are fantastic as well as tech support during the initial set up of the instrument.

Experimental Design and Results Summary

Application

Western Blots, Gels

Starting Material

None

Protocol Overview

Standard western blotting protocol, except the gel needs to be activated by the system prior to transfer.

Tips

Keep the system off when not in use so the camera doesn't get too hot

Results Summary

You get really nice clear images of westerns and regular gels with this imaging system.

Features Summary

Multiple imaging capabilities including fluorescence

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Quality of image and analysis data

The Bad

Optimization

The Bottom Line

This really is a great product. Make sure to have a training on it prior to use and have a blot ready for imaging during that time so you can check out the analysis features.

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