Excellent for Immunocytochemistry

May 16, 2014

Overall

Quality of Results

Ease-of-Optimization

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Cardiovascular Innovation Institute
University of Louisville
Research Coordinator

Company:

Dako

Product Name:

Polyclonal Rabbit anti-GFAP antibody

Catalog Number:

Z0334

Browse Similar Products: GFAP Immunohistochemistry Antibodies

Tried this antibody to stain some mouse oligodendrocyte precursor cells that we differentiated into astrocytes. Works beautifully!

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Primary culture of mouse oligodendrocyte precursor

Primary Incubation

1:500 overnight in 4C

Blocking Agent

PBS + 0.5% BSA + 10% Normal Donkey Serum + 0.1% Triton

Secondary Incubation

1:200 in FITC Donkey anti-Rabbit for 1 hour at room temp

Tertiary Incubation

None

Detection

Immunofluorescence microscopy

Results Summary

Antibody stained GFAP+ astrocytes beautifully, with no non-specific binding in negative controls or isotype controls.

Additional Notes

None

Related Categories

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Summary

The Good

Ease of use, specificity

The Bad

None

The Bottom Line

Excellent antibody for detecting astrocytes

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