Mouse TNF-alpha ELISA MAX™ Standard

Overall

Quality of Results

Ease-of-Optimization

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UCLA
Dept of Surgery
Assist Rescher

Company:

BioLegend

Product Name:

Mouse TNF-α ELISA MAX™ Standard

Catalog Number:

430901

We used this product to detect TNF-α production in LPS activated macrophage culture. It is an easy way to set up this traditional measure system.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Supernatant from LPS activated macrophage culture

Protocol Overview

Very quick and useful kit

Tips

Incubate sample at 37C for 2 hours

Results Summary

This kit provided a perfect standard line. LPS (10ng/ml) activated macrophages for various times, and harvested culture supernatant for ELISA measurement.

Additional Notes

Wash is very important.

Image Gallery

Summary

The Good

High sensitivity

The Bad

The highest concentration of standard line is only 500pg/ml

The Bottom Line

It is a good product, cheap, but provides very nice measurement.

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