Cytocell
FLI1/EWSR1 Aquarius Pathology FISH Probe
LPS 007
I am using the Cytocell FLI1/EWSR1 probe to assess gain or loss of these target regions in lymphoma samples. The probe can also be used to detect translocations between the two regions, as indicated by duel fusion yellow signal.
FISH for gain and loss
Fresh-frozen imprint slides
The probe was applied directly to the slides, which were incubated for 75C for 2 minutes followed by 37C for 24 hours. Slides were then washed in 0.4xSSC with tween followed by 2xSSC and DAPI applied. The EWSR1 probe is labeled in green and the ATM probe is labeled in red. The probe can be used to look at gain or loss of these regions as well as translocations, as indicated by duel fusion (yellow).
DAPI is also supplied
Clear red and green signals, with two copies of each per cell indicating normal status
None
Bright and specific FISH probes
Relatively expensive
Reliable probe that indicates gain/loss of two regions simultaneously as well as translocations.
Product Name: FLI1/EWSR1 Aquarius Pathology FISH ProbeSupplier Page