Santa Cruz Biotechnology, Inc.
Anti-MBD1 (G-18)
sc-9395
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The purpose of this experiment was to identify an antibody that was suitable for detection of MBD1 in human cells by indirect immunofluorescence.
Immunofluorescence
Human hTERT-BJ1 cells
1:200 at room temperature for 60 minutes. Staining was performed in 1 x PBS and 0.1% Tween 20
Slide was pre blocked with 3% BSA in 1 x PBS and 0.1% Tween 20
1:100 dilution of FITC Donkey anti-Goat secondary diluted in 1 x PBS and 0.1% Tween-20 for 60 minutes at room temperature
No tertiary incubation was performed
Direct labeled Donkey anti-Goat antibodies
This antibody detects a strong nuclear signal and shows intense nuclear foci that are characteristic of MBD1 distribution in human cells.
Cells were grown directly on slides and washed briefly with 1 x PBS before fixing and extracting for 10 minutes at room temperature in 4% formaldehyde, 1 x PBS and 0.1% Triton-X100
The signal was strong and as expected for the distribution of this protein
No drawbacks were evident with this antibody
This is a good antibody for immunofluorescence.