Santa Cruz Biotechnology, Inc.
HDAC7 (H-273)
sc-11421
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The purpose of this experiment was to identify an antibody that was suitable for detection of HDAC7 in human cells by indirect immunofluorescence.
Immunofluorescence
Human hTERT-RPE1 cells
1:100 at room temperature for 60 minutes. Staining was performed in 1 x PBS and 0.1% Tween 20
Slide was pre blocked with 3% BSA in 1 x PBS and 0.1% Tween 20
1:100 dilution of FITC Goat anti-rabbit secondary diluted in 1 x PBS and 0.1% Tween-20 for 60 minutes at room temperature
No tertiary incubation was performed
Direct labeled goat anti-rabbit antibodies
This antibody detects a strong cytoplasmic and weak nuclear protein as expected for HDAC7.
Cells were grown directly on slides and washed briefly with 1 x PBS before fixing and extracting for 10 minutes at room temperature in 4% formaldehyde, 1 x PBS and 0.1% Triton-X100
Works well by immunofluorescence.
No drawbacks were evident with this antibody
This antibody detects a protein with a distribution expected for HDAC7 and therefore is suitable for anti-HDAC7 immunofluorescence.