BD Biosciences
Anti-CTCF Mouse IgG1
612148
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Browse Similar Products: CTCF Immunofluorescence Antibodies
The purpose of this experiment was to identify an antibody that was suitable for detection of CTCF in human cells by indirect immunofluorescence.
Immunofluorescence
Human 293T cells
1:100 at room temperature for 60 minutes. Staining was performed in 1 x PBS and 0.1% Tween 20
Slide was pre blocked with 3% BSA in 1 x PBS and 0.1% Tween 20
1:100 dilution of Cy5 Goat anti-mouse secondary diluted in 1 x PBS and 0.1% Tween-20 for 60 minutes at room temperature
No tertiary incubation was performed
Direct labeled goat anti-mouse antibodies
The antibody stains well and detects a nuclear protein. Background is slightly high and sometimes 1:50 dilution is better than 1:100 depending on the cell type.
Cells were grown directly on slides and washed briefly with 1 x PBS before fixing and extracting for 10 minutes at room temperature in 4% formaldehyde, 1 x PBS and 0.1% Triton-X100
The signal is relatively strong, but with some background
No drawbacks were evident with this antibody
This antibody is suitable for immunofluorescence.