Cell Signaling
Acetylated-Lysine Antibody
#9441
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Browse Similar Products: Acetylated-Lysine Western Blot Antibodies
Our Research center contributes to Molecular Neurobiology questions. To measure PTM (post-translational modification) in cells, lots of assays have been developed. Western blotting is one of the easy ways to measure the modification. Here, I showed the simple method to detect modification of protein by acetylation.
Western Blot
Whole cell lysate
1/1000 dilution of antibody. 4oC for overnight incubation
5% skim milk
1/5000 dilution of antibody. 4oC for 2 hr
N/A
HRP
It showed more acetylation in treated (HDAC inhibitor) group compared to control group
None
It is sensitive and good quality antibody. It shows specific acetylated proteins
No
It could detect specific acetylated protein in a simple way.