Staining for Mfn 2 in neural stem cells

November 21, 2014

Overall

Quality of Results

Ease-of-Optimization

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Cell Biology and Neuroscience
University of California, Riverside
Graduate student

Company:

Abcam

Product Name:

Anti mitofusin 2 antibody

Catalog Number:

ab56889

Info: View Product View Product Specs

Browse Similar Products: Mfn 2 Antibodies

Anti Mfn-2 was used to detect effect of environmental toxicants on mitochondrial fusion in stem cells.  This antibody worked well and the labeling obtained was clean after allowing tritox X permeablization of paraformaldehyde fixed cells.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Paraformaldehyde fixed neaural stem cells

Primary Incubation

1:50 in 5% serum, overnight at 4 degrees Celsius

Blocking Agent

5% serum

Secondary Incubation

1:200 for 1 hour at room temperature

Tertiary Incubation

NA

Detection

Nikon TI Eclipse microscope

Results Summary

The antibody works well if a permeablization step with Triton X is incorporated into the protocol.

Additional Notes

None

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Summary

The Good

Clean staining and enough quantity of antibody provided

The Bad

Nothing except the additional permeablization step. Technical support was not very helpful.

The Bottom Line

Good antibody, but the technical support is not very helpful, so other sources of information will be needed.

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