Excellent RAGE Antibody, Works Everytime

April 30, 2014

Overall

Quality of Results

Ease-of-Optimization

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Pharmaceutical Sciences
North Dakota State University
Research Assistant

Company:

Thermo Scientific

Product Name:

RAGE Antibody

Catalog Number:

PA1-84173

We aim to check the expression level of RAGE in neonatal rat brain.  We just selected this antibody as it was the one having cheaper price and specific cross re-activity we intended.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Rat brain

Primary Incubation

1:400

Blocking Agent

Goat serum

Secondary Incubation

1:200

Tertiary Incubation

None

Detection

Fluorescence microscopy

Results Summary

We got nice expression of RAGE in hippocampus area of rat brain

Additional Notes

None

Related Categories

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Summary

The Good

Works everytime

The Bad

Polyclonal antibody

The Bottom Line

I will always use the same antibody for RAGE applications

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